RCMS Research Chair in Meat Safety

William Thériault

Assesment of the development of resistance to colistin in Escherichia coli O149 in pigs

Colistin is a veterinary drug use in swine production to treat post-weaning diarrhea cause by E. coli in piglet. During my graduate study, I studied the bacterial resistance to colistin in E.coli. Using molecular biology technique at the cutting edge of technology, such as gene sequencing, I was able to study mechanism of resistance, the acquisition of this resistance and also I was able to create in vitro bacteria with resistance to colistin. Comparing resistant and sensitive bacteria to this antibiotic, I was able to identify specific mutation in specific DNA gene leading to colistin resistance and contributed to better understand this resistance. The antibiotic resistance is a phenomenon that increases and becoming major issues in veterinary and human medicine. This preoccupation requires special attention from all health professional.
 
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Masters in veterinary science,
animal health and food safety
option

Supervisor: Ann Letellier

Co-supervisors: Marie Archambault and Sylvette Laurent-Lewandowski

Academic Background : Bachelor's degree in biology, microbiology option (2011)

Scientific publications:

Scientific articles

  1. M. Rhouma, F. Beaudry, W. P. Thériault, N. Bergeron, S. Laurent-Lewandowski, J.M. Fairbrother, A. Letellier. Resistance to colistin: What is the fate for this antibiotic in pig production? International Journal of Antimicrobial agents. (accepted)
  2. Rhouma, M. F. Beaudry, W. Thériault, N. Bergeron, S. L.-Lewandowski, JM Fairbrother, A. Letellier. Gastric stability and oral bioavailability of colistin sulphate in pigs challenged or not with Escherichia coli F4 (K88). Research in Veterinary Science 2015 Oct;102:173-81. Doi: 10.1016/j.rvsc.2015.08.005. Epub 2015 Aug 9.
  3. M. Rhouma, F. Beaudry, W. P. Thériault, N. Bergeron, S. Laurent-Lewandowski, J.M. Fairbrother, A. Letellier. In vivo therapeutic efficacy and pharmacokinetics of colistin sulfate in an experimental model of enterotoxigenic Escherichia coli infection in weaned pigs. Veterinary Research.

Published abstracts (conference proceedings)

  1. M. Rhouma, F. Beaudry, W. Thériault, N. Bergeron, S. Laurent-Lewandowski, J.M. Fairbrother, A. Letellier. Impact of colistin sulfate on fecal Escherichia coli resistance and on growth performance of piglets in a post-weaning diarrhea model. Symposium international Safepork 2015. Porto, Portugal. 7-10 septembre 2015. Pages 361-365.
  2. Rhouma, Mohamed, W. P. Thériault, N. Bergeron, S. L.-Lewandowski, J.M. Fairbrother, F. Beaudry, A. Letellier. Oral colistin sulfate in pigs: pharmacokinetics and effect on fecal Escherichia coli excretion in weaned pigs challenged with enterotoxigenic Escherichia coli F4 (K88). Safepork 2013. Portland, Maine, États-Unis. 9 au 12 septembre 2013. Pages 199-202.
  3. Thériault P. William, S. L.-Lewandowski, M. Archambault, A. Letellier. In vitro characterization of colistin resistance and transfert of neomycin resistance in Escherichia coli 0149 strains. ). Safepork 2013. Portland, Maine, États-Unis. 9 au 12 septembre 2013. Pages 192-194.
  4. M. Rhouma, F. Beaudry, W.P. Thériault, N. Bergeron, S. L.-Lewandowski, J.M. Fairbrother, A. Letellier. The effect of colistin sulphate on fecal Escherichia coli excretion and growth performance of weaned pigs challenged with enterotoxigenic Escherichia coli F4 (K88). 8e Symposium du CRIPA, St-Hyacinthe, Québec. 21-22 mai 2015.
  5. Rhouma, Mohamed, W. P. Thériault, N. Bergeron, S. Laurent-Lewandowski, J.M. Fairbrother, F. Beaudry et A. Letellier. Effet de la colistine sulfate sur l’excrétion fécale d’Escherichia coli dans un modèle d’infection expérimentale F4 (K88) chez le porcelet. 7e symposium du CRIPA. St-Hyacinthe, Québec, 3 et 4 juin 2014. 
  6. Thériault P. William, S. L.-Lewandowski, M. Archambault, A. Letellier. In vitro characterization of colistin resistance and transfer of neomycin resistance in Escherichia coli O149 strains. Congrès de l’ACFAS. 6e Symposium du CRIPA. Québec 6-10 mai 2013. 
  7. Rhouma, Mohamed, W. Thériault, S. Laurent-Lewandowski, F. Beaudry et A. Letellier. Étude in vivo du métabolisme de la colistine sulfate chez le porc sain. Journée de la recherche 2012. St-Hyacinthe, Québec. 17 octobre 2012.

Posters

  1. Rhouma Mohamed, Beaudry Francis , Thériault William, Fairbrother John Morris, Letellier Ann. Colistin sulfate exerts a selective pressure on E. coli population during experimental post-weaning diarrhea treatment. Symposium du CRIBIQ sur la biosécurité et les alternatives aux antibiotiques en production animale. Orford, Canada, 15 - 16 mars 2016.
  2. M. Rhouma, F. Beaudry, W. Thériault, N. Bergeron, S. Laurent-Lewandowski, J.M. Fairbrother, A. Letellier. Impact of colistin sulphate on fecal Escherichia coli resistance and on growth performance of piglets in a post-weaning diarrhea model. Symposium international Safepork 2015. Porto, Portugal, 7-10 septembre 2015.  Pages 361-365.
  3. W. Thériault, M. Rhouma, S. L.-Lewandowski, M. Archambault, A. Letellier. Investigation of PmrA/PmrB polymorphism leading to colistin sulphate resistance in E. Coli O149 strain in vitro and in a clinical model of post-weaning diarrhea in piglets. Third international Congress pathogens at the human-animal interface (ICOPHAI 2015). Chiang Mai, Thaïlande. 6 au 8 août 2015.
  4. Thériault P., William, N. Bergeron, M. Rhouma, A. Letellier. Étude comparative des produits homologués chez le porc au Canada et dans les autres pays producteurs de porcs. 7e symposium du CRIPA. St-Hyacinthe, Québec, 3 et 4 juin 2014.
  5. Rhouma, Mohamed, W. P. Thériault, N. Bergeron, S. L.-Lewandowski, J.M. Fairbrother, F. Beaudry, A. Letellier. Oral colistin sulfate in pigs: pharmacokinetics and effect on fecal Escherichia coli excretion in weaned pigs challenged with enterotoxigenic Escherichia coli F4 (K88). Safepork 2013. Portland, Maine, États-Unis. 9 au 12 septembre 2013. (1er prix étudiant)
  6. Thériault P. William, S. L.-Lewandowski, M. Archambault, A. Letellier. In vitro characterization of colistin resistance and transfert of neomycin resistance in Escherichia coli 0149 strains. ). Safepork 2013. Portland, Maine, États-Unis. 9 au 12 septembre 2013.
  7. Thériault P. William, M. Rhouma, S. L.-Lewandowski, M. Archambault, A. Letellier. Study of therapeutics strategies for piglets postweaning diarrhea: in vitro characterization of the colistin outcome in digestive tract, resistance and transfert of neomycin resistance in E. coli 0149 strains. Symposium international ARAE, Ghent, Belgique. 30 juin au 3 juillet 2013.
  8. Rhouma, Mohamed, W. P. Thériault, N. Bergeron, S.L.-Lewandowski, J.M. Fairbrother, F. Beaudry, A. Letellier. Effect of colistin sulphate on fecal Escherichia coli excretion and global health of weaned pigs challenged with Escherichia coli F4 (K88). Congrès de l’ACFAS. 6e Symposium du CRIPA. Québec 6-10 mai 2013.
  9. Rhouma, M., W. Thériault, S. Laurent-Lewandowski, F. Beaudry, A. Letellier. In vitro evaluation of the activity of colistin sulfate and it potential metabolites against Escherichia coli. 5e Symposium du CRIP. St-Hyacinthe, Québec. 16 et 17 mai 2012.

 


2015 October  

Graduated in Master in veterinary science, animal health and food safety option

 

Current situation: Research Officer at RCMS

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