RCMS Research Chair in Meat Safety

Mohamed Rhouma

In vivo assessment of therapeutic efficacy, resistance and pharmacokinetics of colistin sulfate in the treatment of E. coli post weaning diarrhea in pigs 

After weaning, piglets are often infected by Enterotoxigenic Escherichia coli (ETEC), which causes severe diarrhea leading to weight loss and significant mortality. ETEC: F4 isolates have shown a high frequency of resistance to several antibiotics. However, this bacterium is characterized by a low prevalence of resistance against colistin. Even though that colistin is not approved in pigs in Canada, but it is used for the treatment of intestinal infection caused by E. coli in pigs. However, colistin is classified in the category of antibiotics of very high importance in humans. The aim of my project was to assess whether or not the use of colistin was appropriate in pigs. Results: Despite that colistin was effective in the reduction of E. coli fecal shedding it did not prevent weight loss of infected pigs. Conclusion: The use of CS in pig as a first therapeutic choice should be avoided.
 
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Postdoctorat

Supervisor: Ann Letellier

Co-supervisors: Francis Beaudry and Sylvette Laurent-Lewandowski

Academic background :

PhD in veterinary science (2016)

Master in veterinary science,
pharmacology option (2012)

Diploma of Veterinary Medicine (Tunisia, 2009)

 

Awards:

  • 2017: Bourse Mitacs (bourse pour l’élaboration de politiques scientifiques canadiennes) for postdoctoral training at CFIA
  • 2016 : Bourse de rédaction de la Faculté de médecine vétérinaire
  • Septembre 2015 : Bourse de fin d’études doctorales de la FÉSP
  • 2015, September : Bourse de congrès du Centre de Recherche en Infectiologie Porcine et Avicole (CRIPA) 
  • 2013, September : Prix de meilleure présentation par affiche Safepork, Portland, Maine USA (2013, September 9-13th)
  • 2013, January : Bourse d’excellence de 2e année de doctorat en sciences vétérinaires, Faculté de médecine vétérinaire de l’Université de Montréal
  • 2011, September : Bourse d’excellence d’admission au doctorat en sciences vétérinaires

Scientific publications:

Scientific articles

Communications

Guest lecturer

  • Rhouma M, Letellier A, Fravalo P. Rôle de la médecine vétérinaire dans le concept application à la gestion de l'antibiorésistance. Premier Congrès de l’Ecole Nationale de Médecine Vétérinaire de Sidi Thabet-Tunisie, le 23 et 24 mars 2017.
  • Rhouma M. Impcat sur la santé publique de l’utilisation des antibiotiques en production animale. Journée scientifique vétérianire sur l’amélioration de la qualité et l’hygiène du lait. Union général de médecins vétérianires tunisien-Bizerte-Tunisie-30 mars 2017.
  • Rhouma M, Letellier A. Évaluation de l’efficacité thérapeutique et de la résistance à la colistine sulfate lors du traitement de la diarrhée colibacillaire post sevrage chez le porc. Journée annuelle sur l’antibio-surveillance (JAAS), organisée par le Ministère de l'Agriculture, des Pêcheries et de l'Alimentation du Québec (MAPAQ). 23 novembre 2016 - FMV Saint-Hyacinthe.

Oral communications

  1. Rhouma M., F. Beaudry, W. Thériault, J.M. Fairbrother , A. Letellier. Use of colistin sulfate for the oral treatment of experimental post-weaning diarrhea in pigs: Therapeutic efficacy and antibiotic resistance. 6th Clinical Microbiology Conference, 2016, October 20-22th,  Rome, Italy.
  2. Rhouma M., F. Beaudry, W. Thériault, J.M. Fairbrother , A. Letellier. Comparison of efficacy and pharmacokinetics of two oral colistin sulfate doses used for the treatment of experimental post-weaning diarrhea in pigs: (9e Symposium du CRIPA, FMV, Saint-Hyacinthe, 2016, May 25-26th.
  3. Rhouma M., F. Beaudry, W. Thériault, N. Bergeron, J.M. Fairbrother , A. Letellier. The effect of colistin sulfate on fecal Escherichia coli excretion and growth performance of weaned pigs challenged with enterotoxigenic Escherichia coli F4 (K88) (8e Symposium du CRIPA, FMV Saint-Hyacinthe. 2015, May 21-22th.
  4. Rhouma M., F. Beaudry, W. Thériault, N. Bergeron, S. Laurent-Lewandowski, J.M. Fairbrother , A. Letellier. Effect of colistin sulfate on fecal shedding of Escherichia coli and performance of weanling pigs challenged with E. coli F4 (K88). 7th CRIPA Symposium, FMV, Saint-Hyacinthe, 2014, June 3-4th.
  5. Rhouma M., F. Beaudry, W. Thériault, N. Bergeron, S. Laurent-Lewandowski, J.M. Fairbrother , A. Letellier. Étude in vitro et in vivo du métabolisme de la colistine sulfate chez le porc sain. Journée de la recherche, Hôtel des Seigneurs Saint-Hyacinthe, 2012, October 17th.

Posters

  1. Rhouma M., F. Beaudry, W. Thériault, J.M. Fairbrother , A. Letellier. Colistin sulfate exerts a selective pressure on E. coli population during experimental post-weaning diarrhea treatment. Symposium du CRIBIQ sur la biosécurité et les alternatives aux antibiotiques en production animale. 2016, March 15-16th ,  Hôtel Estrimont, Orford, Quebec.
  2. Rhouma M., F. Beaudry, W. Thériault, J.M. Fairbrother , A. Letellier. Impacts of colistin sulfate on fecal Escherichia coli resistance and on growth performance of piglets in a post-weaning diarrhea model. Safepork, Porto, Portugal. 2015, September 7-10th.
  3. W. Thériault, M. Rhouma, S. L.-Lewandowski, M. Archambault, A. Letellier. Investigation of PmrA/PmrB polymorphism leading to colistin sulphate resistance in E. Coli O149 strain in vitro and in a clinical model of post-weaning diarrhea in piglets. Third international Congress pathogens at the human-animal interface (ICOPHAI 2015). Chiang Mai, Thaïlande. 2015, August 6-8th .
  4. Thériault P., William, N. Bergeron, M. Rhouma, A. Letellier. Étude comparative des produits homologués chez le porc au Canada et dans les autres pays producteurs de porcs. 7e symposium du CRIPA. St-Hyacinthe, Québec, 2014, June 3-4th.
  5. Rhouma M., F. Beaudry, W. Thériault, J.M. Fairbrother , A. Letellier. Oral colistin sulfate in pigs: pharmacokinetics and effect on fecal Escherichia coli excretion of weaned pigs challenged with Escherichia coli F4 (K88). Safepork, Portland, Maine, USA; 2013, September 9-12th . (1er prix étudiant)
  6. Thériault P. William, M. Rhouma, S. L.-Lewandowski, M. Archambault, A. Letellier. Study of therapeutics strategies for piglets postweaning diarrhea: in vitro characterization of the colistin outcome in digestive tract, resistance and transfert of neomycin resistance in E. coli 0149 strains. Symposium international ARAE, Ghent, Belgique. 2013, June 30th-July 3th.
  7. Rhouma M., F. Beaudry, W. Thériault, N. Bergeron, S. Laurent-Lewandowski, J.M. Fairbrother , A. Letellier. Effect of colistin sulfate on fecal Escherichia coli and state health of weaned pigs challenged with Escherichia coli F4 (K88). 81e Congrès de l’Acfas. 2013, May 8-9th, Université Laval.
  8. Rhouma M., F. Beaudry, W. Thériault, N. Bergeron, S. Laurent-Lewandowski, J.M. Fairbrother , A. Letellier. In vitro evaluation of the activity of colistin sulfate and it potential metabolites against Escherichia coli. 5e Symposium annuel du CRIPA, FMV Saint-Hyacinthe. 2012, May 16-17th .

 


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December 2016 

Graduate  PhD

 

Current situation : postdoctoral researcher at RCMS                     

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