RCMS Research Chair in Meat Safety

Scientific Articles

Screening for fecal presence of colistin-resistant Escherichia coli and mcr-1 and mcr-2 genes in camel-calves in southern Tunisia
Mohamed Rhouma, S. Bessalah, I. Salhi, William Thériault, John Morris Fairbrother, Philippe Fravalo 
Acta Vet Scand. 2018; 60(1):35

Production and characterization of anti-Campylobacter jejuni IgY derived from egg yolks
Alexandre Thibodeau, Philippe Fravalo, Audrey Perron, Sylvette Laurent-Lewandowski, Ann Letellier
Acta Vet Scand. 2017 Dec 6;59(1):80 doi: 10.1186/s 13028-017-0346-4.

Reduction of Salmonella Shedding by sows during gestation in relation to its fecal microbiome
Guillaume Larivière-Gauthier, Alexandre Thibodeau, Ann Letellier, Évelyne Yergeau, Philippe Fravalo
Front Microbiol. 2017 Nov 10;8:2219. Doi: 10.3389/fmicb.2017.02219

Selection of risk factors to be included in the Canadian Food Inspection Agency risk assessment inspection model for food establishments
Romina Zanabria, Manon Racicot, M. Cormier, Julie Arsenault, Cécile Ferrouillet, Ann Letellier, A. Tiwari, A. Mackay, M. Griffiths, R. Holley, T. Gill, Sylvain Charlebois, Sylvain Quessy
Journal of Food Microbiology. 2018 Oct;75:72-81. doi: 10.1016/j.fm.2017.09.019. Epub 2017 Sep

Phenotypic and Transcriptomic Responses of Campylobacter jejuni suspended in an Artificial Freshwater medium
Hana Trigui, K. Lee, Alexandre Thibodeau, S. Lévesque, N. Mendis, Philippe Fravalo, Ann Letellier, Sébactien Faucher
Front Microbiol. 2017 Sep 20;8:1781. Doi : 10.3389/fmicb.2017.01781.

Recurring Necrotic Enteritis Outbreaks in commercial broiler chicken flocks strongly influence toxin gene carriage and species richness in the resident Clostridium perfringens population
Marie-Lou Gaucher, Gabriel Perron, Julie Arsenault, Ann Letellier, Martine Boulianne, Sylvain Quessy
Front Microbiol. 2017 May 17;8:881. Doi: 10.3389/fmicb.2017.00881

Nutrient limitation and-DNA : two elements required for a stable knitted network – like structure in Listeria monocytogenes biofilm
Tamazight Cherifi , Mario Jacques, Sylvain Quessy, Philippe Fravalo
Frontiers in Microbiology. 17 may 2017. Doi: 10.3389/fmicb.2017.0086

Characterisation of InlA truncation in Listeria monocytogenes isolates from farm animals and human cases in the province of Quebec
Philippe Fravalo, Tamazight Cherifi, Kersti Neira, Ann Letellier, John M. Fairbrother, Sadia Bekal
Vet record open. Apr 2017, 4 (1) e000199; DOI: 10.1136/vetreco-2016-000199.

Lack of Evidence that Selenium-yeat improves chicken health and modulates the caecal microbiota in the context of lonication by Campylobacter jejuni
AlexandreThibodeau, Ann Letellier, Evelyne Yergeau, Guillaume Larivière-Gauthier, Philippe Fravalo
Frontiers in Microbiology. 17 mars 2016.Doi:10.3389/fmicb.2017.00451

Dynamics of virus distribution in a defined swine production network using enteric viruses as molecular markers
Virginie Lachapelle, Ann Letellier, Philippe Fravalo, Julie Brassard, Yvan L’Homme
Appl Environ microbial. 2017 Feb 1;83(4). pii: e03187-16 doi: 10.1128/AEM.03187-16

Post weaning diarrhea in pigs: Risk factors and non-colistin-based control strategies.
Mohamed Rhouma, John M. Fairbrother , Francis Beaudry, Ann Letellier
2017.Acta. Vet. Scand. 59:31.

Extended-spectrum β-lactamase, carbapenemase, and the mcr-1 gene: Is there a historical link?
Mohamed Rhouma, Ann Letellier
Int. J. Antimicrob. 49: 269-271. 2017.

The fecal presence of enterotoxin and F4 genes as an indicator of efficacy of treatment with colistin sulfate in pigs
Mohamed Rhouma, John M. Fairbrother, William Thériault, Francis Beaudry, Nadia Bergeron, Sylvette Laurent-Lewandowski, Ann Letellier
BMC Microbiol. 17: 1-7. 2017.

Feed presentation options in swine early fattening mitigates Salmonella shedding and specifically modulates the faecal microbiota
Philippe Lebel, Ann Letellier, Jessie Longpré, Benoît Laplante, Evelyne Yergeau, Philippe Fravalo
J Appl Mircobiol. 2017 Jan;122(1) :30-39. Doi : 10.1111/jam.13305

Colistin in pig production: Chemistry, Mechanism of antibacterial action, Resistance emergence, and One Health Perspectives.
Mohamed Rhouma
, Francis Beaudry, William Thériault, Ann Letellier
Front. Microb. 7:1-22. 2016.  

Dynamics of extended-spectrum cephalosporin-resistance in pathogenic Escherichia coli isolated from diseased pigs in Quebec, Canada
Seyedehameneh Jahanbakhsh, Ann Letellier, John M. Fairbrother
International Journal of Antimicrobial Agents. Août 2016

Detection and phylogenetic analysis of the Hepatitis E virus in a Canadian swine production network
Nicolas Nantel-Fortier
, Ann Letellier, Virginie Lachapelle, Philippe Fravalo, Yvan L’Homme
Food and Environmental Virology. 15 July 2016

In vivo therapeutic efficacy and pharmacokinetics of colistin sulfate in an experimental model of enterotoxigenic Escherichia coli infection in weaned pigs
Mohamed Rhouma, Francis Beaudry, William Thériault, Nadia Bergeron, Sylvette Laurent-Lewandowski, John M. Fairbrother, Ann Letellier
Vet. Res. 47:58. Mai 2016

Impact of mash feeding versus pellets on propionic/butyric acid levels and on total Escherichia coli load in the gastrointestinal tract of growing pigs
Jessie Longpré, John M. Fairbrother, Philippe Fravalo, Julie Arsenault, Philippe LeBel, Benoît Laplante, Charles Surprenant, Daniel Massé, Ann Letellier
Journal of Animal Science. 19 février 2016

Resistance to colistin: what is the fate for this antibiotic in pig production?
Mohamed Rhouma, Francis Beaudry, Ann Letellier
International Journal of Antimicrobial Agents. Avril 2016. doi: 10.1016/j.ijantimicag.2016.04.008

Survival in water of Campylobacter jejuni strains isolated from the slaughterhouse
Hana Trigui, Alexandre Thibodeau, Philippe Fravalo, Ann Letellier and Sébastien P. Faucher
SpringerPlus. December 2015, 4:799

Persistence of Indicator and Pathogenic Microorganisms in Broccoli Following Manure Spreading and Irrigation with E. coli-contaminated Water: Field Experiment
Mylène Généreux, Marie-Jo Breton, John M. Fairbrother, Philippe Fravalo, Caroline Côté
Journal of Food Protection October 2015, pp. 1776-1784

Gastric stability and oral bioavailability of colistin sulfate in pigs challenged or not with Escherichia coli O149: F4 (K88)
Mohamed Rhouma, Francis Beaudry, William Thériault, Nadia Bergeron, Sylvette Laurent-Lewandowski, John Morris Fairbrother, Ann Letellier
Research in Veterinary Science 102. October 2015. 173–181

Chicken caecal microbiome modifications induced by Campylobacter jejuni colonization and by a non-antibiotic feed additive
Alexandre Thibodeau , Philippe Fravalo, Étienne Yergeau, Julie Arsenault, Ludovic Lahaye, Ann Letellier
PLoS One. 10 juillet 2015; 10(7): e0131978

Different stainless-steel types for equipment do not affect the initial microbial transfer from pork skin including pathogens
Guillaume Larivière-Gauthier, Sylvain Quessy, S. Fournaise, Ann Letellier, Philippe Fravalo
Canadian Journal of Veterinary Research. Can J Vet Res. 2015 Jul;79(3):255-9.

Extensive characterization of Campylobacter jejuni chicken isolates to uncover genes involved in the ability to compete for gut colonization
Alexandre Thibodeau, Philippe Fravalo, Eduardo N. Taboada, Sylvette Laurent-Lewandowski, Évelyne Guévremont, Sylvain Quessy, Ann Letellier
BMC Microbiology, 2015 May 10;15:97.

Analysis of Listeria monocytogenes strains distribution in a pork slaughter and cutting plant in the province of Quebec
Guillaume Larivière-Gauthier, Ann Letellier, Annaelle Kérouanton, Sadjia Bekal, Sylvain Quessy, Sylvain Fournaise, Philippe Fravalo
Journal of food protection. Number 12, December 2014, pp. 2012-2218, pp. 2121-2128(8)

Modification of Campylobacter jejuni broiler colonization through an experimental blend of encapsulated organic acids and essential oils used as feed additive
Alexandre Thibodeau, Philippe Fravalo, R. Gauthier, Évelyne Guévremont, Nadia Bergeron, Sylvette Laurent-Lewandowski, Sylvain Quessy, Martine Boulianne, Ann Letellier
Journal of agricultural Science and Technology A & B. 20 octobre 2014. pp. 853-864

Assessing most practical and effective protocols to sanitize hands of poultry catching crew members
Manon Racicot, A. Kocher, G. Beauchamp, Ann Letellier, J.-P. Vaillancourt
Preventive Veterinary Medicine. PREVET 2013 Aug 1;111(1-2):92-9)

Drug use and resistance among E.coli and Enterococcus isolates from chicken and turkey flocks slaughtered in Québec, Canada
Martine Boulianne, Julie Arsenault, D. Daigneault, Marie Archambault, Ann Letellier, Lucie Dutil
Can J Vet Res. 2016 Jan; 80(1): 49–59
Genetic diversity of group A rotavirus in swine in Canada
Virginie Lachapelle
, J.-S. Sohal, M.-C. Lambert, J. Brassard, Philippe Fravalo, Ann Letellier, Y. L’Homme
2014 Jan 9.Arch Virol.
Effect of low dose of fumonisins on pig health: immune status, intestinal microbiota and sensitivity to Salmonella
Christine Burel, M. Tanguy, P. Guerre, E. Boilletot, R. Cariolet, M. Quéguiner, G. Postollec, P. Pinton, G. Salvat, I.P. Oswald, P. Fravalo
Toxins (Basel). 2013 Apr 23;5(4): 841-64
Distribution of Colonization and Antimicrobial Resistance Genes in Campylobacter jejuni Isolated from Chicken
Alexandre Thibodeau, Philippe Fravalo, P. Garneau, L. Masson, Sylvette Laurent-Lewandowski, Sylvain Quessy, Josée Harel, Ann Letellier
Foodborne Pathog Dis. 2013 Apr;10(4):382-91. doi: 10.1089/fpd.2012.1271. Epub 2013 Mar 19.
Capillary electrophoresis single-strand conformation polymorphism for the monitoring of gastrointestinal microbiota of chicken flocks
Christine Pissavin, C. Burel, I. Gabriel, V. Beven, S. Mallet, R. Maurice, M. Quéguiner, M. Lessire, P. Fravalo
Poult Sci. 2012 Sep;91(9): 2294-304
Antimicrobial-Resistant Enterococcus faecalis in Abattoir Pigs and Plasmid Colocalization and Cotranfer of tet(M) and erm(B) Genes.
Cindy-Love Tremblay, Ann Letellier, Sylvain Quessy, Danielle Daignault et Marie Archambault
Journal of Food Protection, 2012, Vol. 75(9):1595–1602
Systemic inflammation and priming of peripheral blood leukocytes persist during clinical remission in horses with heaves
Anouk Lavoie-Lamoureux, Guy Beauchamp, Sylvain Quessy, James G. Martin, Jean-Pierre Lavoie
Veterinary Immunology and Immunopathology, 146 (2012) 35– 45
Campylobacter from sows in farrow-to-finish pig farms ; risk indicators and genetic diversity
M. Denis, E. Henrique, B. Chidaine, A. Tircot, S. Bougeard, P. Fravalo
Vet. Microbiol., 2011 dec. 29; 154(1-2):163-70
Presence and characterization of Campylobacter jejuni in organically raised chickens in Quebec
Alexandre Thibodeau
, Philippe Fravalo, Sylvette Laurent-Lewandowski, Evelyne Guévremont, Sylvain Quessy, Ann Letellier
The Canadian Journal of Veterinary Research, 2011;75:298–307
Analysis of Staphylococcus enterotoxin B using differential isotopic tags and liquid chromatography quadrupole ion trap mass spectrometry
Khanh Dang Bao, Ann Letellier and Francis Beaudry.
Biomedical chromatography, octobre 2011, on line article
Multiple-Antibiotic Resistance of Enterococcus faecalis and Enterococcus faecium from caecal contents in broiler chicken and turkey flocks slaughtered in Québec, Canada, and plasmid co-localization of tetO and ermB genes.
Cindy-Love Tremblay, Ann Letellier, Sylvain Quessy, Danielle Daignault, Martine Boulianne , Marie Archambault
Journal of Food Protection. 2011 Oct;74(10): 1639-48.
Description and sources of contamination by Campylobacter spp. of river water destined for human consumption in Britanny, France
M. Denis, M. tanguy, B. Chidaine, Laisney M.J., F. Mégraud , P. Fravalo
Pathol. Biol. (Paris), 2011 oct; 59(5):256-63
Risk factor for Listeria monocytogenes contamination in French laying hens and broiler flocks
K. Aury, S. Le Bouquin, M.T. Toquin, A. Huneau-Salaün, Y. Le Nôtre, V. Allain, I. Petetin, P. Fravalo, M. Chemaly
M. Prev. Vet. Med., 2011 mars 1;98(4):271-8
Continuous feeding of antimicrobial growth promoters to commercial swine during the growing ⁄ finishing phase does not modify faecal community erythromycin resistance or community structure
M. Kalmokoff, L.M. Waddington, M. Thomas, K.-L. Liang, C. Ma, E. Topp, U. Desranleau Dandurand, A. Letellier, F. Matias and S.P.J. Brooks
Journal of Applied Microbiology, mars 2011 (110), 1414–1425
Knowledge and Attitudes toward Food Safety and Use of Good Production Practices among Canadian Broiler Chicken Producers
Young I., Rajic A., Letellier A., Cox B., Leslie M., Sanei B., A. Mcewen S.
Journal of Food Protection, Vol. 73, No. 7, 2010, Pages 1278–1287
A simplified method for detecting pathogenic Yersinia enterocolitica in slaugntered pig tonsils
M. Fondrevez, A. Labbé, E. Houard, P. Fravalo, F. Madec, M. Denis
J. Microbiol. Methods, 2010 nov.;83(2):244-9
Immune response following vaccination against Salmonella Enteritidis using 2 commercial bacterins in laying hens
Q.L. Tran Thi, Quessy S., Letellier A., Desrosiers A., Boulianne M.
The Canadian Journal of Veterinary Research, 2010; 74:185–192
Characterization of Salmonella Typhimurium isolates associated withsepticemia in swine
Bergeron N., Corriveau J., Letellier A., Daigle F., Quessy Q.
The Canadian Journal of Veterinary Research, 2010; 74:11–17
Presence of zoonotic pathogens in physico-chemically characterized manures from hog finishing houses using different production systems
Létourneau V., Dichaine C., Côté C., Letellier A., Topp E., Massé D.
Bioresource Technology 101, 2010, 4048–40
Quantitative and qualitative evaluation of Campylobacter spp. contamination of turckey caecal contents and carcasses during and following the slaughtering process
L. Bily, J. Petton, F. Lalande, S. Rouxel, M. denis, M. Chemaly, G. Salvat, P. Fravalo
J. Food Prot., 2010, 73(7):1212-18
Interaction between Host Cells and Septicemic Salmonella enterica serovar Typhimurium isolates from pigs
Bergeron N., Corriveau J., Letellier A., Daigle F., Lessard L., Quessy S.
Journal of clinical microbiology, Nov. 2009, p. 3413–3419 Vol. 47, No. 11
Isolation of Salmonella enterica in laying-hen flocks and assesment of eggshell contamination in France
M. Chemaly, A. Huneau-Salaün, A. Labbé, C. Houdayer, I. Petetin, P. Fravalo
J. Food Prot., 2009 oct; 72(10):2071-77
Campylobacter transfer from naturally contamined chicken thighs to cutting boards is inversely related to initial load
P. Fravalo, M.J. Laisney, M.O. Gillard, G. Salvat, M. Chemaly
J. Food Prot., 2009 sep; 72(9):1836-40
Risk factors for Salmonella enterica subsp. enterica contamination in 519 French laying hen flocks at the end of the laying period
A. Huneau-Salaün, M. Chemaly, S. Le Bouquin, F. Lalande, I. Petetin, S. Rouxel, V. Michel, P. Fravalo, N. Rose
Preventive Veterinary Medecine, 2009; 89:51-58
Risk Factors at Slaughter Associated with Presence of Salmonella on Hog Carcasses in Canada
Letellier A., Beauchamp G., Guévremont E., D’allaire S., Hurnik D., Quessy S.
Journal of Food Protection, Vol. 72, No. 11, 2009, Pages 2326–2331
Impact of Deoxynivalenol on the Intestinal Microflora of Pigs
Yann J. Waché, Charlotte Valat, Gilbert Postollec, Stephanie Bougeard, Christine Burel, Isabelle P. Oswald and Philippe Fravalo 
Int J Mol Sci. Jan 2009; 10(1): 1–17
Comparative analysis of different TaqMan real-time RT-PCR assays for the detection of swine Hepatitis E virus and integration of Feline calicivirus as internal control
P. Ward, E. Poitras, D. Leblanc, A. Letellier, J. Brassard, D. Plante and A. Houde
Journal of Applied Microbiology, janvier 2009 (106), 1360–1369

Detection by TaqMan real-time RT-PCR and molecular characterization of swine Hepatitis E virus
Ward, P., É. Poitras, D. Leblanc, A. Letellier, S. Quessy, J. Brassard, A. Houde
Can J. Vet Res. 2008.72 : (4) 311-9

A Reservoir of Drug-Resistant Pathogenic Bacteria in Asymptomatic Hosts
G. Perron, S. Quessy and G. Bell
PLoS ONE. 2008; 3(11): e3749

Evidence of cross-contamination by Campylobacter spp. of broiler carcasses using genetic characterization of isolates
Valérie Normand, Martine Boulianne, Sylvain Quessy
Can J Vet Res. 2008 Oct;72(5):396-402

Parallel evolution of multidrug-resistance in Salmonella enterica isolated from swine
Gabriel Perron, G. Bell, Sylvain Quessy
FEMS Microbiol Lett. 2008 Apr;281(1):17-22
Comparison of the selection of antimicrobial resistance in fecal Escherichia coli during enrofloxacin administration with a local drug delivery system or with intramuscular injections in a swine model
Béraud R., Huneault L., Bernier D., Beaudry F., Letellier A, R.E. Del Castillo J.
The Canadian Journal of Veterinary Research, 2008; 72:311–319
Antibiotic resistance in Escherichia coli and Enterococcus spp. isolates from commercial broiler chickens receiving growth-promoting doses of bacitracin or virginiamycin
Thibodeau A., Quessy S., Guévremont É., Houde A., Topp E., Sory Diarra M., Letellier A.
The Canadian Journal of Veterinary Research, 2008; 72:129–136
Molecular characterization of Hepatitis E virus detected in swine farms in the province of Quebec
Ward P., Müller P., Letellier A, Quessy S., Simard C., Trottier Y.-L., Houde A., Brassard J.
The Canadian Journal of Veterinary Research, 2008; 72:27–31
Cecal Populations of Lactobacilli and Bifidobacteria and Escherichia coli Populations After In Vivo Escherichia coli Challenge in Birds Fed Diets with Purified Lignin or Mannanoligosaccharides
B. Baurhoo, A. Letellier, X. Zhao, C. A. Ruiz-Feria
Poultry Science 2007 dec; 86(12):2509-16 
Prevalence and risk factors for Salmonella spp. and Campylobacter spp. caecal colonization in broiler chicken
and turkey flocks slaughtered in Quebec, Canada

Arsenault J., Letellier A., Quessy S, Normand V, Boulianne M.
Preventive Veterinary Medicine 81 (2007) 250–264
Prevalence and risk factors for Salmonella spp. and Campylobacter spp. caecal colonization in broiler chicken
and turkey flocks slaughtered in Quebec, Canada

Arsenault J., Letellier A., Quessy S., Normand V., Boulianne M.
Preventive Veterinary Medicine 81 (2007) 250–264
Genotypic diversity and antimicrobial resistance in asymptomatic Salmonella enterica serotype Typhimurium DT104
Perron G., Quessy S., Letellier A., Bell G.
Infection, Genetics and Evolution 7 (2007) 223–228
Prevalence and Risk Factors for Salmonella and Campylobacter spp. Carcass Contamination in Turkeys Slaughtered in Quebec, Canada
Arsenault J., Letellier A., Quessy S., Morin J.-P., Boulianne M.
Journal of Food Protection, Vol. 70, No. 6, 2007, 1350–1359