Laboratory and Services

PRODUCTS AND SERVICES OFFERED BY GREMIP AND CRIPA

Serological diagnosis - Bacteriological diagnosis - Production of biological materials -

Veterinary consultations - Services on demand - Products offered -

*** Services and expertise of CRIPA ***
 

 
 

SEROLOGICAL DIAGNOSIS :


- ELISA-BASED DETECTION OF ANTIBODIES AGAINST :

 

BACTERIOLOGICAL DIAGNOSIS :


- SEROTYPING :

- DETECTION, IDENTIFICATION AND CHARACTERIZATION OF MICROORGANISMS :

Actinobacillus pleuropneumoniae

Detection by coagglutination in lungs
Detection with magnetic beads in tonsils

Detection by PCR

Identification and profil of cytokines by PCR
Genotypic characterization of the strains by RAPD

Escherichia coli

From infected tissues, PCR-based detection of:
- diarrhoeal factors: STa, STb, LT, Eae, VT1(SLTI) and VT2(SLTII)
- septicaemic factors: CNF, P(pap), aerobactin and Tsh
- adhesins: F4(K88), F5(K99), F6(987P), F41 and F18(F107)

Haemophilus parasuis

Genotypic characterization of strains by RAPD

Mycoplasma hyopneumoniae

Selective isolation and identification (in progress)

Pasteurella multocida

ELISA- and PCR-based detection of toxin in nasal swabs or directly from the bacterial strain
   

Streptococcus suis

Detection in tonsils using magnetic beads or PCR
Genotypic characterization of the strains by RAPD

Brachypsira hyodysenteriae and Brachypsira pilosicoli

PCR-based detection in feces
Identification of the strain using PCR
Genotypic characterization of the strains by RAPD

Lawsonia intracellularis

PCR-based detection in formalin-fixed tissues and in feces

 

- ALSO OFFERED: 

  • Determination of the minimal inhibitory concentration (MIC)
  • Characterization of microorganisms using transmission and scanning electron microscopy.
 

PRODUCTION OF BIOLOGICAL MATERIALS :


  • PCR-based detection for the virulence factors of pathogenic E. coli
  • Bacterial lysates using French-Press
  • Lyophilization of large volume (litres)
  • Vaccine trials against colibacillosis, pleuropneumonia, and Streptococcus suis infections
  • Serum-specific serotyping of A. pleuropneumoniae, E. coli, and S. suis
 

VETERINARY CONSULTATIONS :


  • On swine diseases and management of swine herds
  • For research protocols using pig as a model
  • Use of the management program Pig CHAMP
 

SERVICES THAT COULD BE OFFERED ON DEMAND


  • Development of rapid diagnostic tools (ELISA)
  • Production and purification of monoclonal and polyclonal antibodies
  • Peptide and protein purification
  • Affinity chromatography (pending the availability of ligand)
  • Immunogold labelling for transmission electron microscopy
  • Cloning and sequence analysis
  • Development of PCR-based and RAPD-based diagnostic tools
  • Experiments using gnotobiotic piglets
  • Development of vaccines
  • Model and infrastructure for experimental infections
  • Establish infectious dose for experimental infections
  • Production of fusion and recombinant proteins
 

PRODUCTS OFFERED BY THE GREMIP (Enquire about full price list)


Serotyping of Escherichia coli

Antisera Pool 1N (serogroups of neonatal diarrhoea)
Antisera Pool 2N (serogroups of postweaning diarrhoea)

Antisera K88 (F4)

Antisera K99 (F5)

Serotyping of Streptococcus suis

Antisera against serotypes 1 à 34 (including 1/2)

Serotyping of Actinobacillus pleuropneumoniae

Antisera against serotypes 1 to 12

 

SERVICES AND EXPERTISES OF CRIPA