Laboratory & Services

PRODUCTS AND SERVICES OFFERED BY GREMIP

- Serological diagnosis - Bacteriological diagnosis - Biological materials - Veterinary consultations - On demand - Products -

SEROLOGICAL DIAGNOSIS : - ELISA-BASED DETECTION OF ANTIBODIES AGAINST :

• Actinobacillus pleuropneumoniae (Reference laboratory)
• Escherichia coli (Reference laboratory)
• Brachypsira hyodysenteriae
• Mycoplasma hyopneumoniae

BACTERIOLOGICAL DIAGNOSIS :

- SEROTYPING :

• Actinobacillus pleuropneumoniae (Reference laboratory)
• Escherichia coli (Reference laboratory)
• Streptococcus suis (Reference laboratory)

- DETECTION, IDENTIFICATION AND CHARACTERIZATION OF MICROORGANISMS :

Actinobacillus pleuropneumoniae	Detection by coagglutination in lungs
	Detection with magnetic beads in tonsils
	Detection by PCR
	Identification and profil of cytokines by PCR
	Genotypic characterization of the strains by RAPD
Escherichia coli	From infected tissues, PCR-based detection of:
	- diarrhoeal factors: STa, STb, LT, Eae, VT1(SLTI) and VT2(SLTII)
	- septicaemic factors: CNF, P(pap), aerobactin and Tsh
	- adhesins: F4(K88), F5(K99), F6(987P), F41 and F18(F107)
Haemophilus parasuis	Genotypic characterization of strains by RAPD
Mycoplasma hyopneumoniae	Selective isolation and identification (in progress)
Pasteurella multocida	ELISA- and PCR-based detection of toxin in nasal swabs or directly from the bacterial strain
Streptococcus suis	Detection in tonsils using magnetic beads or PCR
	Genotypic characterization of the strains by RAPD
Brachypsira hyodysenteriae and Brachypsira pilosicoli	PCR-based detection in feces
	Identification of the strain using PCR
	Genotypic characterization of the strains by RAPD
Lawsonia intracellularis	PCR-based detection in formalin-fixed tissues and in feces

- ALSO OFFERED:

• Determination of the minimal inhibitory concentration (MIC)
• Characterization of microorganisms using transmission and scanning electron microscopy.

PRODUCTION OF BIOLOGICAL MATERIALS :

• PCR-based detection for the virulence factors of pathogenic E. coli
• Bacterial lysates using French-Press
• Lyophilization of large volume (litres)
• Vaccine trials against colibacillosis, pleuropneumonia, and Streptococcus suis infections
• Serum-specific serotyping of A. pleuropneumoniae, E. coli, and S. suis

VETERINARY CONSULTATIONS :

• On swine diseases and management of swine herds
• For research protocols using pig as a model
• Use of the management program Pig CHAMP

SERVICES THAT COULD BE OFFERED ON DEMAND

• Development of rapid diagnostic tools (ELISA)
• Production and purification of monoclonal and polyclonal antibodies
• Peptide and protein purification
• Affinity chromatography (pending the availability of ligand)
• Immunogold labelling for transmission electron microscopy
• Cloning and sequence analysis
• Development of PCR-based and RAPD-based diagnostic tools
• Experiments using gnotobiotic piglets
• Development of vaccines
• Model and infrastructure for experimental infections
• Establish infectious dose for experimental infections
• Production of fusion and recombinant proteins

PRODUCTS OFFERED BY THE GREMIP
(enquire about full price list)

Serotyping of Escherichia coli	Antisera Pool 1N (serogroups of neonatal diarrhoea)
	Antisera Pool 2N (serogroups of postweaning diarrhoea)
	Antisera K88 (F4)
	Antisera K99 (F5)
Serotyping of Streptococcus suis	Antisera against serotypes 1 à 34 (including 1/2)
Serotyping of Actinobacillus pleuropneumoniae	Antisera against serotypes 1 to 12