Members

THE ACTINOBACILLUS PLEUROPNEUMONIAE LABORATORY

- Research team - Field of activities - Research themes - Achievements - Publications -

 

Principal investigator:  Dr Mario Jacques

Telephone:                 450-773-8521, office 8348 or laboratory 8272

Fax:                          450-778-8108

E-mail:                      This email address is being protected from spambots. You need JavaScript enabled to view it.

MjacqueslaboWeb251010

Research Assistant:

  • Josée Labrie, M.Sc

Postdoctoral Fellow:

  • Yannick Tremblay
    Since September 2009

Ph.D Students:

  • Audrey Charlebois
    Supervision: Dr. Marie Archambault
    Since January 2010

M.Sc Students:

  • Alexandra Grasteau
    Since September 2008
  • Cynthia Lévesque
    Co-supervision: Dr. Carl A. Gagnon
    Since September 2008
  • Chan (Ashley) Wu
    Co-supervision: Dr. Michaël Mourez
    Since September 2010

 

FIELD OF ACTIVITIES

  • Identification and characterization of the virulence factors of A. pleuropneumoniae

 

RESEARCH THEMES

Molecular analysis of virulence factors of Actinobacillus pleuropneumoniae involved in respiratory tract colonization

  • Role of lipopolysaccharides (LPS) in bacterial adherence
  • Characterization and mutagenesis of genes involved in biofilm formation

Actinobacillus pleuropneumoniae in vivo-expressed genes

  • Transcriptomic profiles using DNA microarrays
  • Development of a mucosal sub-unit vaccine

 

ACHIEVEMENTS

  • In vitro model for the study of bacterial adherence to cells of the swine respiratory tract
  • First comparative genomic hybridizations and transcriptomic profiling in A. pleuropneumoniae
  • Development of microtiter plate and flow cell assays to study biofilm formation

 

PUBLICATIONS (past 6 years)

2010-2009-2008-2007-2006-2005

 2010

Jacques, M., V. Aragon, and Y.D.N. Tremblay. 2010. Biofilm formation in bacterial pathogens of veterinary importance. Animal Health Research Reviews (in press).

Ster, C., F. Beaudoin, M.S. Diarra, M. Jacques, F. Malouin, P. Lacasse. 2010. Evaluation of some Staphylococcus aureus iron–regulated proteins as vaccine targets. Veterinary Immunology and Immunopathology 136:311-318.

Silversides, D.W., N. Music, M. Jacques, C.A. Gagnon, R. Webby. 2010. Investigation of the species origin of the St. Jude Porcine Lung epithelial cell line (SJPL) made available to researchers. Journal of Virology 84:5454-5455.

Deslandes, V., M. Denicourt, C. Girard, J. Harel, J.H.E. Nash, M. Jacques. 2010.  Transcriptional profiling of Actinobacillus pleuropneumoniae during the acute phase of a natural infection in pigs. BMC Genomics 11:98.

Labrie, J., G. Pelletier-Jacques, V. Deslandes, M. Ramjeet, É. Auger, J.H.E. Nash, M. Jacques. 2010. Effects of growth conditions on biofilm formation by Actinobacillus pleuropneumoniae. Veterinary Research 41:3.

2009

Lone, A.G., V. Deslandes, J.H.E. Nash, M. Jacques, J.I. MacInnes. 2009. malT knockout mutation invokes a stringent type gene-expression profile in Actinobacillus pleuropneumoniae in bronchoalveolar fluid. BMC Microbiology 14:195.

Lone, A.G., V. Deslandes, J.H.E. Nash, M. Jacques, J.I. MacInnes. 2009. Modulation of gene expression in Actinobacillus pleuropneumoniaeexposed to bronchoalveolar fluid. PLoS ONE 4·(7):e6139. doi:10.1371/journal.pone.0006139.

Gouré, J., W.A. Findlay, V. Deslandes, A. Bouevitch, S.J. Foote, J.I. MacInnes, J.W. Coulton, J.H.·Nash, M. Jacques. 2009. Microarray–based comparative genomic profiling of reference strains and selected Canadian field isolates of Actinobacillus pleuropneumoniae. BMC·Genomics 10:88.

Bouchet, B., G. Vanier, M. Jacques, É. Auger, M. Gottschalk. 2009. Studies on the interactions of Haemophilus parasuis with porcine epithelial tracheal cells: Limited role of LOS in apoptosis and pro–inflammatory cytokine release. Microbial Pathogenesis 46:108-13.

Auger, É., V. Deslandes, M. Ramjeet, I. Contreras, J.H. Nash, J. Harel, M. Gottschalk, M.·Oliver and·M. Jacques. 2009. Host–pathogen interaction of Actinobacillus pleuropneumoniae with porcine lung and tracheal epithelial cells. Infection and Immunity 77:1426-41.· Publication sélectionnée pour la section «·Journal Highlights·» de la revue Microbe de l’ASM (mai 2009).

2008

Ramjeet, M., A.·D. Cox, M.·A. Hancock, M.·Mourez, J. Labrie, M.·Gottschalk and·M. Jacques. 2008. Mutation in the LPS outer core biosynthesis gene, galU, affects LPS interaction with the RTX toxins ApxI and ApxII and cytolytic activity of Actinobacillus pleuropneumoniaeserotype·1. Molecular Microbiology 70:221–235.

Ramjeet, M., V. Deslandes, J. Gouré, M. Jacques. 2008. Actinobacillus pleuropneumoniae vaccines: from bacterins to new insights into vaccination strategies. Animal Health Research Reviews 9:25–45.

Bouchet, B., G. Vanier, M. Jacques, M. Gottschalk. 2008. Interactions of Haemophilus parasuis and its LOS with porcine brain microvascular endothelial cells. Veterinary Research 39:42. 

2007

Chung, W.J., C. Ng–Thow–Hing, L.IL Budman, B.F. Gibbs, J. H.E. Nash, M. Jacques, and J.W.·Coulton. 2007. Outer membrane proteome of Actinobacillus pleuropneumoniae:LC-MS/MS analyses validate in silico predictions. Proteomics 7:1854–1865.

Deslandes, V., J.H.E. Nash, J. Harel, J.W. Coulton and M. Jacques. 2007. ·Transcriptional profiling of Actinobacillus pleuropneumoniae under iron–restricted conditions. BMC·Genomics 8:72.

2006

Allard, M., H. Moisan, E. Brouillette, A.L. Gervais, M. Jacques, P. Lacasse, M.S. Diarra and F. Malouin. 2006. Transcriptional modulation of some Staphylococcus aureus iron–regulated genes during growth in vitro and in a tissue cage model in vivo. Microbes and Infection 8:1679–1690.

Lagacé, L. M. Jacques, A.A. Mafu, and D. Roy. 2006. Biofilm formation and biocides sensitivity of Pseudomonas marginalis isolated from a maple sap collection system. Journal of Food Protection 69:2411–2416.

Shakarji, L., L. G. Mikael, R. Srikumar, M. Kobisch, J. W. Coulton and M. Jacques. 2006. FhuA and HgbA, Outer Membrane Proteins of Actinobacillus pleuropneumoniae: Role as Virulence Determinants. Canadian Journal of Microbiology 52:391–396.

Lillie, B.N., J.D. Hammermueller, J.I. Macinnes, M. Jacques, M.A. Hayes. 2006. Porcine mannan–binding lectin A binds to Actinobacillus suis and Haemophilus parasuis. Developmental and Comparative Immunology 30:954–965.

Lagacé, L., M. Jacques, A. A. Mafu and D. Roy. 2006. Compositions of maple sap microflora and collection system biofilms evaluated by scanning electron microscopy and denaturing gradient gel electrophoresis. International Journal of Food Microbiology 109:9–18.

2005

Li, Y., G. Martinez, M. Gottschalk, S. Lacouture, P. Willson, J.D. Dubreuil, M. Jacques and J. Harel. 2005. Identification of a Surface Protein of Streptococcus suis and Evaluation of its Immunogenic and Protective Capacity in Pigs. Infection and Immunity 74:305–312.

Jacques, M., J. Labrie, F. St. Michael, A. D. Cox, A.-M. Paradis, C.P. Dick, C. Klopfenstein, A. Broes, N. Fittipaldi and M. Gottschalk. 2005. Isolation of an Atypical Strain of Actinobacillus pleuropneumoniae Serotype 1 With a Truncated Lipopolysaccharide Outer Core and No O–Antigen. Journal of Clinical Microbiology. 43:3522-3525.

Vanier, G., A. Szczotka, P. Friedl, S. Lacouture, M. Jacques and M. Gottschalk. 2005. Haemophilus parasuis invades porcine brain microvascular endothelial cells. Microbiology 152:135–142.

Ramjeet, M., V. Deslandes, F. St. Michael, A. D. Cox, M. Kobisch, M. Gottschalk and M. Jacques. 2005. Truncation of the LPS outer core affects susceptibility to antimicrobial peptides and virulence of Actinobacillus pleuropneumoniae serotype 1. Journal of Biological Chemistry 280:39104–39114.